› 1paq

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC FRAGMENT OF EUKARYOTIC INITIATION FACTOR 2B EPSILON

Released:
DOI: 10.2210/pdb1paq/pdb
PDB entry 1paq coloured by chain, front view.
PDB entry 1paq coloured by chain, side view.
PDB entry 1paq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the catalytic fragment of translation initiation factor 2B and identification of a critically important catalytic residue.
Boesen T, Mohammad SS, Pavitt GD, Andersen GR
J. Biol. Chem. 279 10584-92 (2004)
PMID: 14681227

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Translation initiation factor eIF2B subunit epsilon Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 22.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32501 (Residues: 524-712; Coverage: 27%)
Gene names: GCD6, TIF225, YD8142.12, YD8142B.03, YDR211W
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: I422
Unit cell:
a: 106.34Å b: 106.34Å c: 91.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.243 0.272
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Translational regulation of GCN4 and the general amino acid control of yeast.
Hinnebusch AG. (2005)
9 more

6 mentions without citation

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae.
Dever et al. (2016)
5 more