› 1pgz

X-ray diffraction
2.6Å resolution

Crystal Structure of UP1 Complexed With d(TTAGGGTTAG(6-MI)G); A Human Telomeric Repeat Containing 6-methyl-8-(2-deoxy-beta-ribofuranosyl)isoxanthopteridine (6-MI)

Released:
DOI: 10.2210/pdb1pgz/pdb
PDB entry 1pgz coloured by chain, front view.
PDB entry 1pgz coloured by chain, side view.
PDB entry 1pgz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based incorporation of 6-methyl-8-(2-deoxy-beta-ribofuranosyl)isoxanthopteridine into the human telomeric repeat DNA as a probe for UP1 binding and destabilization of G-tetrad structures.
Myers JC, Moore SA, Shamoo Y
J Biol Chem 278 42300-6 (2003)
PMID: 12904298

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114377 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09651 (Residues: 2-196; Coverage: 52%)
Gene names: HNRNPA1, HNRPA1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-D(*T*TP*AP*GP*GP*GP*TP*TP*AP*GP*(6MI)P*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.82 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 51.622Å b: 51.622Å c: 171.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
1 more

8 mentions without citation

Idiosyncrasies of hnRNP A1-RNA recognition: Can binding mode influence function.
Levengood et al. (2019)
7 more