› 1pjh

X-ray diffraction
2.1Å resolution

Structural studies on delta3-delta2-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily

Released:
DOI: 10.2210/pdb1pjh/pdb
PDB entry 1pjh coloured by chain, front view.
PDB entry 1pjh coloured by chain, side view.
PDB entry 1pjh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily.
Mursula AM, Hiltunen JK, Wierenga RK
FEBS Lett 557 81-7 (2004)
PMID: 14741345

Function and Biology Details

Reaction catalysed: 
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,2-trans-enoyl-CoA isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 280 amino acids
Theoretical weight: 31.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05871 (Residues: 1-280; Coverage: 100%)
Gene names: ECI1, YLR284C
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 10 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P41212
Unit cell:
a: 116.243Å b: 116.243Å c: 216.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.189
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Peroxisomal beta-oxidation--a metabolic pathway with multiple functions.
Poirier et al. (2006)
1 more

4 mentions without citation

Using sequence similarity networks for visualization of relationships across diverse protein superfamilies.
Atkinson et al. (2009)
3 more