› 1pl7

X-ray diffraction
2.2Å resolution

Human Sorbitol Dehydrogenase (apo)

Released:
DOI: 10.2210/pdb1pl7/pdb
PDB entry 1pl7 coloured by chain, front view.
PDB entry 1pl7 coloured by chain, side view.
PDB entry 1pl7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase.
Pauly TA, Ekstrom JL, Beebe DA, Chrunyk B, Cunningham D, Griffor M, Kamath A, Lee SE, Madura R, Mcguire D, Subashi T, Wasilko D, Watts P, Mylari BL, Oates PJ, Adams PD, Rath VL
Structure 11 1071-85 (2003)
PMID: 12962626

Function and Biology Details

Reactions catalysed: 
L-iditol + NAD(+) = L-sorbose + NADH
(R,R)-butane-2,3-diol + NAD(+) = (R)-acetoin + NADH
Ribitol + NAD(+) = D-ribulose + NADH
Xylitol + NAD(+) = D-xylulose + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorbitol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 356 amino acids
Theoretical weight: 38.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00796 (Residues: 2-357; Coverage: 100%)
Gene name: SORD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P62
Unit cell:
a: 134.946Å b: 134.946Å c: 224.686Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
2 more

7 mentions without citation

Zinc-binding cysteines: diverse functions and structural motifs.
Pace et al. (2014)
6 more