› 1pt9

X-ray diffraction
2.42Å resolution

Crystal Structure Analysis of the DIII Component of Transhydrogenase with a Thio-Nicotinamide Nucleotide Analogue

Released:
DOI: 10.2210/pdb1pt9/pdb
PDB entry 1pt9 coloured by chain, front view.
PDB entry 1pt9 coloured by chain, side view.
PDB entry 1pt9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation.
Singh A, Venning JD, Quirk PG, van Boxel GI, Rodrigues DJ, White SA, Jackson JB
J Biol Chem 278 33208-16 (2003)
PMID: 12791694

Function and Biology Details

Reaction catalysed: 
NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-171675 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P) transhydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 22.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13423 (Residues: 880-1086; Coverage: 19%)
Gene name: NNT
Sequence domains: NAD(P) transhydrogenase beta subunit
Structure domains: TPP-binding domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand TAP 2 x TAP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P4122
Unit cell:
a: 57.4Å b: 57.4Å c: 251.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.278

Quick links

Citations

4 citation in other articles

Differences in a conformational equilibrium distinguish catalysis by the endothelial and neuronal nitric-oxide synthase flavoproteins.
Ilagan et al. (2008)
3 more