› 1ptt

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH PHOSPHOTYROSINE-CONTAINING TETRA-PEPTIDE (AC-DEPYL-NH2)

Released:
DOI: 10.2210/pdb1ptt/pdb
PDB entry 1ptt coloured by chain, front view.
PDB entry 1ptt coloured by chain, side view.
PDB entry 1ptt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for phosphotyrosine peptide recognition by protein tyrosine phosphatase 1B.
Jia Z, Barford D, Flint AJ, Tonks NK
Science 268 1754-8 (1995)
PMID: 7540771

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148266 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
PHOSPHOTYROSINE-CONTAINING TETRA-PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 645 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P3121
Unit cell:
a: 88.5Å b: 88.5Å c: 124.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

64 review citations

Free radicals in the physiological control of cell function.
Dröge W. (2002)
63 more

5 mentions without citation

Protein tyrosine phosphatases: structure, function, and implication in human disease.
Tautz et al. (2013)
4 more