› 1pzu

X-ray diffraction
3.1Å resolution

An asymmetric NFAT1-RHR homodimer on a pseudo-palindromic, Kappa-B site

Released:
DOI: 10.2210/pdb1pzu/pdb
PDB entry 1pzu coloured by chain, front view.
PDB entry 1pzu coloured by chain, side view.
PDB entry 1pzu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An asymmetric NFAT1 dimer on a pseudo-palindromic kappa B-like DNA site.
Jin L, Sliz P, Chen L, Macián F, Rao A, Hogan PG, Harrison SC
Nat Struct Biol 10 807-11 (2003)
PMID: 12949491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114417 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear factor of activated T-cells, cytoplasmic 2 Chains: B, D, H, I, L, M
Molecule details ›
Chains: B, D, H, I, L, M
Length: 301 amino acids
Theoretical weight: 34.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13469 (Residues: 396-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains:
Structure domains:
5'-D(*TP*TP*GP*AP*GP*GP*AP*AP*TP*TP*TP*CP*CP*A)-3' Chains: T, V, X
Molecule details ›
Chains: T, V, X
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*AP*TP*GP*GP*AP*AP*AP*TP*TP*CP*CP*TP*C)-3' Chains: W, Y, Z
Molecule details ›
Chains: W, Y, Z
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 81.7Å b: 122.9Å c: 241.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.303 0.296 0.319
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

NFAT proteins: key regulators of T-cell development and function.
Macian F. (2005)
8 more

4 mentions without citation

A dimer-specific function of the transcription factor NFATp.
Falvo et al. (2008)
3 more