› 1q0x

X-ray diffraction
1.6Å resolution

Anti-morphine Antibody 9B1 Unliganded Form

Released:
DOI: 10.2210/pdb1q0x/pdb
PDB entry 1q0x coloured by chain, front view.
PDB entry 1q0x coloured by chain, side view.
PDB entry 1q0x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine.
Pozharski E, Wilson MA, Hewagama A, Shanafelt AB, Petsko G, Ringe D
J Mol Biol 337 691-7 (2004)
PMID: 15019787

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134501 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig lambda-1 chain V region Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 22.53 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01723 (Residues: 21-116; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9D9B8 (Residues: 7-104; Coverage: 88%)
Gene names: Gm16747, Igh-VJ558
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 56.514Å b: 59.924Å c: 114.706Å
α: 90° β: 92.36° γ: 90°
R-values:
R R work R free
0.216 0.216 0.251
Expression system: Not provided

Quick links

Citations

18 citation in other articles

Quantification of cation-pi interactions in protein-ligand complexes: crystal-structure analysis of Factor Xa bound to a quaternary ammonium ion ligand.
Schärer et al. (2005)
17 more

3 mentions without citation

Blind prediction performance of RosettaAntibody 3.0: grafting, relaxation, kinematic loop modeling, and full CDR optimization.
Weitzner et al. (2014)
2 more