› 1q79

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF MAMMALIAN POLY(A) POLYMERASE

Released:
DOI: 10.2210/pdb1q79/pdb
PDB entry 1q79 coloured by chain, front view.
PDB entry 1q79 coloured by chain, side view.
PDB entry 1q79 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase.
Martin G, Möglich A, Keller W, Doublié S
J Mol Biol 341 911-25 (2004)
PMID: 15328606

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase alpha Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 59.23 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25500 (Residues: 1-514; Coverage: 70%)
Gene names: PAP, PAPOLA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand 3AT 1 x 3AT
Ligand GOL 5 x GOL
2 modified residues:
Ligand CSD 2 x CSD
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 57.64Å b: 63.07Å c: 181.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
3 more

5 mentions without citation

Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Xiang et al. (2014)
4 more