› 1qdq

X-ray diffraction
2.18Å resolution

X-RAY CRYSTAL STRUCTURE OF BOVINE CATHEPSIN B-CA074 COMPLEX

Released:
DOI: 10.2210/pdb1qdq/pdb
PDB entry 1qdq coloured by chain, front view.
PDB entry 1qdq coloured by chain, side view.
PDB entry 1qdq coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Substrate specificity of bovine cathepsin B and its inhibition by CA074, based on crystal structure refinement of the complex.
Yamamoto A, Tomoo K, Hara T, Murata M, Kitamura K, Ishida T
J Biochem 127 635-43 (2000)
PMID: 10739956

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-|- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin B Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.49 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P07688 (Residues: 80-332; Coverage: 80%)
Gene name: CTSB
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand 074 1 x 074
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 73.06Å b: 73.06Å c: 141.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.183

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Citations

3 review citations

The structural basis of specific protease-inhibitor interactions at the plant-pathogen interface.
Hörger et al. (2013)
2 more

16 mentions without citation

Cathepsin B in neurodegeneration of Alzheimer's disease, traumatic brain injury, and related brain disorders.
Hook et al. (2020)
15 more