› 1qfw

X-ray diffraction
3.5Å resolution

TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT

Released:
DOI: 10.2210/pdb1qfw/pdb
PDB entry 1qfw coloured by chain, front view.
PDB entry 1qfw coloured by chain, side view.
PDB entry 1qfw coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a ternary complex between human chorionic gonadotropin (hCG) and two Fv fragments specific for the alpha and beta-subunits.
Tegoni M, Spinelli S, Verhoeyen M, Davis P, Cambillau C
J Mol Biol 289 1375-85 (1999)
PMID: 10373373

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134115 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Glycoprotein hormones alpha chain Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.22 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01215 (Residues: 25-116; Coverage: 100%)
Gene name: CGA
Sequence domains: Glycoprotein hormone
Structure domains: Cystine-knot cytokines
Choriogonadotropin subunit beta 3 Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 15.55 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0DN86 (Residues: 21-165; Coverage: 100%)
Gene names: CGB, CGB3, CGB5, CGB8
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
Ig kappa chain V-III region PC 3741/TEPC 111 Chain: L
Molecule details ›
Chain: L
Length: 114 amino acids
Theoretical weight: 12.69 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01660 (Residues: 1-100, 101-111; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain V region 102 Chain: H
Molecule details ›
Chain: H
Length: 117 amino acids
Theoretical weight: 12.9 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01750 (Residues: 20-116; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-III region PC 3741/TEPC 111 Chain: M
Molecule details ›
Chain: M
Length: 108 amino acids
Theoretical weight: 11.9 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01660 (Residues: 1-111; Coverage: 96%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain V region 345 Chain: I
Molecule details ›
Chain: I
Length: 122 amino acids
Theoretical weight: 13.46 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P18526 (Residues: 20-117; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 104.9Å b: 104.9Å c: 150.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.26 0.31
Expression system: Not provided

Quick links

Citations

12 review citations

Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships.
Szkudlinski et al. (2002)
11 more

20 mentions without citation

Structural-Functional Features of the Thyrotropin Receptor: A Class A G-Protein-Coupled Receptor at Work.
Kleinau et al. (2017)
19 more