› 1qip

X-ray diffraction
1.72Å resolution

HUMAN GLYOXALASE I COMPLEXED WITH S-P-NITROBENZYLOXYCARBONYLGLUTATHIONE

Released:
DOI: 10.2210/pdb1qip/pdb
PDB entry 1qip coloured by chain, front view.
PDB entry 1qip coloured by chain, side view.
PDB entry 1qip coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Reaction mechanism of glyoxalase I explored by an X-ray crystallographic analysis of the human enzyme in complex with a transition state analogue.
Cameron AD, Ridderström M, Olin B, Kavarana MJ, Creighton DJ, Mannervik B
Biochemistry 38 13480-90 (1999)
PMID: 10521255

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 183 amino acids
Theoretical weight: 20.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04760 (Residues: 2-184; Coverage: 100%)
Gene name: GLO1
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments


Cofactor: Ligand GNB 4 x GNB
2 bound ligands:
Ligand ZN 4 x ZN
Ligand BME 4 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43
Unit cell:
a: 67.3Å b: 67.3Å c: 167.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.18 0.21
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes.
Deponte M. (2013)
15 more

10 mentions without citation

Nonredox nickel enzymes.
Maroney et al. (2014)
9 more