› 1qki

X-ray diffraction
3Å resolution

X-RAY STRUCTURE OF HUMAN GLUCOSE 6-PHOSPHATE DEHYDROGENASE (VARIANT CANTON R459L) COMPLEXED WITH STRUCTURAL NADP+

Released:
DOI: 10.2210/pdb1qki/pdb
PDB entry 1qki coloured by chain, front view.
PDB entry 1qki coloured by chain, side view.
PDB entry 1qki coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human glucose-6-phosphate dehydrogenase: the crystal structure reveals a structural NADP(+) molecule and provides insights into enzyme deficiency.
Au SW, Gover S, Lam VM, Adams MJ
Structure 8 293-303 (2000)
PMID: 10745013

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 514 amino acids
Theoretical weight: 59.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11413 (Residues: 2-515; Coverage: 100%)
Gene name: G6PD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 8 x NAP
2 bound ligands:
Ligand GOA 8 x GOA
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 128.9Å b: 208.7Å c: 214.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.294
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Glucose-6-phosphate dehydrogenase deficiency.
Cappellini et al. (2008)
21 more

21 mentions without citation

On the molecular discrimination between adenine and guanine by proteins.
Nobeli et al. (2001)
20 more