› 1qkl

Solution NMR

hRPABC14.4, essential subunit of human RNA polymerases I, II and III

Released:
DOI: 10.2210/pdb1qkl/pdb
PDB entry 1qkl coloured by chain, ensemble of 22 models, front view.
PDB entry 1qkl coloured by chain, ensemble of 22 models, side view.
PDB entry 1qkl coloured by chain, ensemble of 22 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the hRPABC14.4 subunit of human RNA polymerases.
Gaskell A, Gilbert D, Ladias JA, Wagner G
Nat Struct Biol 6 1039-42 (1999)
PMID: 10542096

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61218 (Residues: 1-127; Coverage: 100%)
Gene names: POLR2F, POLRF
Sequence domains: RNA polymerase Rpb6
Structure domains: Eukaryotic RPB6 RNA polymerase subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY AND SIMULATED ANNEALING
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Dynamic structures of intrinsically disordered proteins related to the general transcription factor TFIIH, nucleosomes, and histone chaperones.
Okuda et al. (2022)
7 other articles

4 mentions without citation

von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination.
Kuznetsova et al. (2003)
3 more