› 1qkt

X-ray diffraction
2.2Å resolution

MUTANT ESTROGEN NUCLEAR RECEPTOR LIGAND BINDING DOMAIN COMPLEXED WITH ESTRADIOL

Released:
DOI: 10.2210/pdb1qkt/pdb
PDB entry 1qkt coloured by chain, front view.
PDB entry 1qkt coloured by chain, side view.
PDB entry 1qkt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a mutant hERalpha ligand-binding domain reveals key structural features for the mechanism of partial agonism.
Gangloff M, Ruff M, Eiler S, Duclaud S, Wurtz JM, Moras D
J Biol Chem 276 15059-65 (2001)
PMID: 11278577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 28.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03372 (Residues: 304-551; Coverage: 42%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE
Spacegroup: P6522
Unit cell:
a: 58.61Å b: 58.61Å c: 276.02Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.273
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Estrogen receptors: how do they signal and what are their targets.
Heldring et al. (2007)
13 more

22 mentions without citation

Versatility or promiscuity: the estrogen receptors, control of ligand selectivity and an update on subtype selective ligands.
Ng et al. (2014)
21 more