› 1qqo

X-ray diffraction
1.9Å resolution

E175S MUTANT OF BOVINE 70 KILODALTON HEAT SHOCK PROTEIN

Released:
DOI: 10.2210/pdb1qqo/pdb
PDB entry 1qqo coloured by chain, front view.
PDB entry 1qqo coloured by chain, side view.
PDB entry 1qqo coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Mapping the role of active site residues for transducing an ATP-induced conformational change in the bovine 70-kDa heat shock cognate protein.
Johnson ER, McKay DB
Biochemistry 38 10823-30 (1999)
PMID: 10451379

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 378 amino acids
Theoretical weight: 41.49 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19120 (Residues: 4-381; Coverage: 58%)
Gene names: HSC70, HSPA8
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand CL 2 x CL
Ligand K 1 x K
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 143.756Å b: 64.029Å c: 46.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
7 more