› 1qsf

X-ray diffraction
2.8Å resolution

STRUCTURE OF A6-TCR BOUND TO HLA-A2 COMPLEXED WITH ALTERED HTLV-1 TAX PEPTIDE Y8A

Released:
DOI: 10.2210/pdb1qsf/pdb
PDB entry 1qsf coloured by chain, front view.
PDB entry 1qsf coloured by chain, side view.
PDB entry 1qsf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Four A6-TCR/peptide/HLA-A2 structures that generate very different T cell signals are nearly identical.
Ding YH, Baker BM, Garboczi DN, Biddison WE, Wiley DC
Immunity 11 45-56 (1999)
PMID: 10435578

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-208585 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 20-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
TAX PEPTIDE Y8A Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 978 Da
T cell receptor alpha chain constant Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
HUMAN T-CELL RECEPTOR Chain: E
Molecule details ›
Chain: E
Length: 243 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 224.665Å b: 48.46Å c: 93.739Å
α: 90° β: 90.46° γ: 90°
R-values:
R R work R free
0.245 0.245 0.286
Expression system: Escherichia coli

Quick links

Citations

70 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
69 more

13 mentions without citation

Modeling the structure of bound peptide ligands to major histocompatibility complex.
Tong et al. (2004)
12 more