› 1r0p

X-ray diffraction
1.8Å resolution

Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met in complex with the microbial alkaloid K-252a

Released:
DOI: 10.2210/pdb1r0p/pdb
PDB entry 1r0p coloured by chain, front view.
PDB entry 1r0p coloured by chain, side view.
PDB entry 1r0p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met and its complex with the microbial alkaloid K-252a.
Schiering N, Knapp S, Marconi M, Flocco MM, Cui J, Perego R, Rusconi L, Cristiani C
Proc Natl Acad Sci U S A 100 12654-9 (2003)
PMID: 14559966

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hepatocyte growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 35.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08581 (Residues: 1049-1360; Coverage: 23%)
Gene name: MET
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand KSA 1 x KSA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 42.969Å b: 46.247Å c: 158.379Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.197
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
25 more

23 mentions without citation

Impact of the Protein Data Bank on antineoplastic approvals.
Westbrook et al. (2020)
22 more