› 1r7f

Solution NMR

NMR structure of the membrane anchor domain (1-31) of the nonstructural protein 5A (NS5A) of hepatitis C virus (Ensemble of 43 structures. Sample in 100mM SDS)

Released:
DOI: 10.2210/pdb1r7f/pdb
PDB entry 1r7f coloured by chain, ensemble of 43 models, front view.
PDB entry 1r7f coloured by chain, ensemble of 43 models, side view.
PDB entry 1r7f coloured by chain, ensemble of 43 models, top view.
Source organism: Hepatitis C virus (isolate H)
Primary publication:
Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A.
Penin F, Brass V, Appel N, Ramboarina S, Montserret R, Ficheux D, Blum HE, Bartenschlager R, Moradpour D
J Biol Chem 279 40835-43 (2004)
PMID: 15247283

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151107 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 5A Chain: A
Molecule details ›
Chain: A
Length: 31 amino acids
Theoretical weight: 3.77 KDa
Source organism: Hepatitis C virus (isolate H)
Expression system: Not provided
UniProt:
  • Canonical: P27958 (Residues: 1973-2003; Coverage: 1%)
Sequence domains: Hepatitis C virus non-structural 5a protein membrane anchor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing, molecular dynamics, energy minimization
Expression system: Not provided

Quick links

Citations

42 review citations

Replication of hepatitis C virus.
Moradpour et al. (2007)
41 more

1 mention without citation

Asymmetric binding to NS5A by daclatasvir (BMS-790052) and analogs suggests two novel modes of HCV inhibition.
Nettles et al. (2014)