› 1rho

X-ray diffraction
2.5Å resolution

STRUCTURE OF RHO GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR

Released:
DOI: 10.2210/pdb1rho/pdb
PDB entry 1rho coloured by chain, front view.
PDB entry 1rho coloured by chain, side view.
PDB entry 1rho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm.
Keep NH, Barnes M, Barsukov I, Badii R, Lian LY, Segal AW, Moody PC, Roberts GC
Structure 5 623-33 (1997)
PMID: 9195882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-156565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GDP-dissociation inhibitor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 145 amino acids
Theoretical weight: 16.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52565 (Residues: 59-203; Coverage: 71%)
Gene names: ARHGDIA, GDIA1
Sequence domains: RHO protein GDP dissociation inhibitor
Structure domains: Coagulation Factor XIII, subunit A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: R32
Unit cell:
a: 156.57Å b: 156.57Å c: 132.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.298
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Rho GTPases and their effector proteins.
Bishop et al. (2000)
14 more

6 mentions without citation

Recommendations towards standards for quantitative MRI (qMRI) and outstanding needs.
Keenan et al. (2019)
5 more