› 1ro4

Solution NMR

RDC-derived models of the zinc ribbon domain of human general transcription factor TFIIB (zinc free structures)

Released:
DOI: 10.2210/pdb1ro4/pdb
PDB entry 1ro4 coloured by chain, ensemble of 35 models, front view.
PDB entry 1ro4 coloured by chain, ensemble of 35 models, side view.
PDB entry 1ro4 coloured by chain, ensemble of 35 models, top view.
Source organism: Homo sapiens
Primary publication:
Probing Zn2+-binding effects on the zinc-ribbon domain of human general transcription factor TFIIB.
Ghosh M, Elsby LM, Mal TK, Gooding JM, Roberts SG, Ikura M
Biochem J 378 317-24 (2004)
PMID: 14641108

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169410 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor IIB Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 6.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00403 (Residues: 1-60; Coverage: 19%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: TFIIB zinc-binding
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The general transcription machinery and general cofactors.
Thomas et al. (2006)
4 more

1 mention without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)