› 1roc

X-ray diffraction
1.5Å resolution

Crystal structure of the histone deposition protein Asf1

Released:
DOI: 10.2210/pdb1roc/pdb
PDB entry 1roc coloured by chain, front view.
PDB entry 1roc coloured by chain, side view.
PDB entry 1roc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and function of the conserved core of histone deposition protein Asf1.
Daganzo SM, Erzberger JP, Lam WM, Skordalakes E, Zhang R, Franco AA, Brill SJ, Adams PD, Berger JM, Kaufman PD
Curr Biol 13 2148-58 (2003)
PMID: 14680630

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone chaperone ASF1 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 2-154; Coverage: 55%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

1 bound ligand:
Ligand BR 9 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 94.395Å b: 31.702Å c: 53.625Å
α: 90° β: 124.55° γ: 90°
R-values:
R R work R free
0.2 0.198 0.246
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Histone chaperones: an escort network regulating histone traffic.
De Koning et al. (2007)
15 more

12 mentions without citation

Histone chaperone networks shaping chromatin function.
Hammond et al. (2017)
11 more