› 1rtf

X-ray diffraction
2.3Å resolution

COMPLEX OF BENZAMIDINE WITH THE CATALYTIC DOMAIN OF HUMAN TWO CHAIN TISSUE PLASMINOGEN ACTIVATOR [(TC)-T-PA]

Released:
DOI: 10.2210/pdb1rtf/pdb
PDB entry 1rtf coloured by chain, front view.
PDB entry 1rtf coloured by chain, side view.
PDB entry 1rtf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 2.3 A crystal structure of the catalytic domain of recombinant two-chain human tissue-type plasminogen activator.
Lamba D, Bauer M, Huber R, Fischer S, Rudolph R, Kohnert U, Bode W
J Mol Biol 258 117-35 (1996)
PMID: 8613982

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133302 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TWO CHAIN TISSUE PLASMINOGEN ACTIVATOR Chain: A
Molecule details ›
Chain: A
Length: 17 amino acids
Theoretical weight: 2.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Tissue-type plasminogen activator chain B Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00750 (Residues: 311-562; Coverage: 47%)
Gene name: PLAT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 59.2Å b: 59.2Å c: 136.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.265
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Developmental roles of HGF/SF and its receptor, the c-Met tyrosine kinase.
Birchmeier et al. (1998)
20 more

7 mentions without citation

Crystal Structure of the Michaelis Complex between Tissue-type Plasminogen Activator and Plasminogen Activators Inhibitor-1.
Gong et al. (2015)
6 more