› 1ryh

X-ray diffraction
1.75Å resolution

Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase

Released:
DOI: 10.2210/pdb1ryh/pdb
PDB entry 1ryh coloured by chain, front view.
PDB entry 1ryh coloured by chain, side view.
PDB entry 1ryh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase.
Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR
J Biol Chem 279 4743-9 (2004)
PMID: 14625275

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-75, 76-182; Coverage: 95%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GNP 2 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 51.55Å b: 78.67Å c: 96.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.219
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged.
David et al. (2010)
25 more

3 mentions without citation

Structural Mechanisms and Drug Discovery Prospects of Rho GTPases.
Smithers et al. (2016)
2 more