› 1s18

X-ray diffraction
1.7Å resolution

Structure and protein design of human apyrase

Released:
DOI: 10.2210/pdb1s18/pdb
PDB entry 1s18 coloured by chain, front view.
PDB entry 1s18 coloured by chain, side view.
PDB entry 1s18 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and protein design of a human platelet function inhibitor.
Dai J, Liu J, Deng Y, Smith TM, Lu M
Cell 116 649-59 (2004)
PMID: 15006348

Function and Biology Details

Reaction catalysed: 
A nucleoside diphosphate + H(2)O = a nucleoside phosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble calcium-activated nucleotidase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 37.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WVQ1 (Residues: 71-401; Coverage: 83%)
Gene names: CANT1, SHAPY
Sequence domains: Apyrase
Structure domains: Apyrase

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ACT 4 x ACT
Ligand TRS 7 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 43.163Å b: 52.501Å c: 77.925Å
α: 99.44° β: 106.58° γ: 99.89°
R-values:
R R work R free
0.159 0.157 0.191
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Nucleotide- and nucleoside-converting ectoenzymes: Important modulators of purinergic signalling cascade.
Yegutkin GG. (2008)
2 more

2 mentions without citation

Cultivating uncultured bacteria from northern wetlands: knowledge gained and remaining gaps.
Dedysh SN. (2011)
1 more