› 1s8o

X-ray diffraction
2.6Å resolution

Human soluble Epoxide Hydrolase

Released:
DOI: 10.2210/pdb1s8o/pdb
PDB entry 1s8o coloured by chain, front view.
PDB entry 1s8o coloured by chain, side view.
PDB entry 1s8o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis.
Gomez GA, Morisseau C, Hammock BD, Christianson DW
Biochemistry 43 4716-23 (2004)
PMID: 15096040

Function and Biology Details

Reactions catalysed: 
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional epoxide hydrolase 2 Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 62.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34913 (Residues: 1-555; Coverage: 100%)
Gene name: EPHX2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand P6G 1 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6522
Unit cell:
a: 92.546Å b: 92.546Å c: 244.637Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.272
Expression system: Spodoptera frugiperda

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Citations

23 review citations

Action of epoxyeicosatrienoic acids on cellular function.
Spector et al. (2007)
22 more

16 mentions without citation

Cytochrome P450 Metabolism of Polyunsaturated Fatty Acids and Neurodegeneration.
Sarparast et al. (2020)
15 more