› 1scf

X-ray diffraction
2.2Å resolution

HUMAN RECOMBINANT STEM CELL FACTOR

Released:
DOI: 10.2210/pdb1scf/pdb
PDB entry 1scf coloured by chain, front view.
PDB entry 1scf coloured by chain, side view.
PDB entry 1scf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the active core of human stem cell factor and analysis of binding to its receptor kit.
Jiang X, Gurel O, Mendiaz EA, Stearns GW, Clogston CL, Lu HS, Osslund TD, Syed RS, Langley KE, Hendrickson WA
EMBO J 19 3192-203 (2000)
PMID: 10880433

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kit ligand Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 273 amino acids
Theoretical weight: 31.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P21583 (Residues: 1-273; Coverage: 100%)
Gene names: KITLG, MGF, SCF
Sequence domains: Stem cell factor
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

2 bound ligands:
Ligand 1PE 1 x 1PE
Ligand CA 3 x CA
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 71.82Å b: 82.55Å c: 88.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.242
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Myocyte renewal and ventricular remodelling.
Anversa et al. (2002)
16 more

9 mentions without citation

Structure of the active core of human stem cell factor and analysis of binding to its receptor kit.
Jiang et al. (2000)
8 more