› 1shz

X-ray diffraction
2.85Å resolution

Crystal Structure of the p115RhoGEF rgRGS Domain in A Complex with Galpha(13):Galpha(i1) Chimera

Released:
DOI: 10.2210/pdb1shz/pdb
PDB entry 1shz coloured by chain, front view.
PDB entry 1shz coloured by chain, side view.
PDB entry 1shz coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator.
Chen Z, Singer WD, Sternweis PC, Sprang SR
Nat Struct Mol Biol 12 191-7 (2005)
PMID: 15665872

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-145468 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein subunit alpha-13 Chains: A, D
Molecule details ›
Chains: A, D
Length: 340 amino acids
Theoretical weight: 39.64 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P10824 (Residues: 22-48, 187-211, 216-231, 243-354; Coverage: 51%)
  • Canonical: P27601 (Residues: 64-208, 234-237, 254-264; Coverage: 42%)
Gene names: Gna-13, Gna13, Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:
Rho guanine nucleotide exchange factor 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 233 amino acids
Theoretical weight: 26.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92888 (Residues: 7-239; Coverage: 26%)
Gene name: ARHGEF1
Sequence domains: Regulator of G protein signalling-like domain
Structure domains: Regulator of G-protein Signalling 4, domain 2

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ALF 2 x ALF
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 199.747Å b: 105.274Å c: 71.748Å
α: 90° β: 96.91° γ: 90°
R-values:
R R work R free
0.229 0.229 0.297
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets.
Kimple et al. (2011)
14 more

8 mentions without citation

Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors.
Aittaleb et al. (2010)
7 more