› 1si3

X-ray diffraction
2.6Å resolution

Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex

Released:
DOI: 10.2210/pdb1si3/pdb
PDB entry 1si3 coloured by chain, front view.
PDB entry 1si3 coloured by chain, side view.
PDB entry 1si3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain.
Ma JB, Ye K, Patel DJ
Nature 429 318-322 (2004)
PMID: 15152257

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193904 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein argonaute-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UL18 (Residues: 225-369; Coverage: 17%)
Gene names: AGO1, EIF2C1
Sequence domains: PAZ domain
Structure domains: paz domain
5'-R(*CP*GP*UP*GP*AP*CP*UP*CP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.81 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P64
Unit cell:
a: 99.799Å b: 99.799Å c: 34.933Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.247 0.304
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

115 review citations

Biogenesis of small RNAs in animals.
Kim et al. (2009)
114 more

11 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
10 more