› 1sir

X-ray diffraction
2.6Å resolution

The Crystal Structure and Mechanism of Human Glutaryl-CoA Dehydrogenase

Released:
DOI: 10.2210/pdb1sir/pdb
PDB entry 1sir coloured by chain, front view.
PDB entry 1sir coloured by chain, side view.
PDB entry 1sir coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human glutaryl-CoA dehydrogenase with and without an alternate substrate: structural bases of dehydrogenation and decarboxylation reactions.
Fu Z, Wang M, Paschke R, Rao KS, Frerman FE, Kim JJ
Biochemistry 43 9674-84 (2004)
PMID: 15274622

Function and Biology Details

Reaction catalysed: 
(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaryl-CoA dehydrogenase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.52 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q92947 (Residues: 45-438; Coverage: 90%)
Gene name: GCDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand NBC 1 x NBC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6422
Unit cell:
a: 116.722Å b: 116.722Å c: 128.034Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.24

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Citations

6 review citations

Anaerobic catabolism of aromatic compounds: a genetic and genomic view.
Carmona et al. (2009)
5 more

11 mentions without citation

Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover.
Nagpal et al. (2006)
10 more