1sj4

X-ray diffraction
2.7Å resolution

Crystal structure of a C75U mutant Hepatitis Delta Virus ribozyme precursor, in Cu2+ solution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-140466 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chain: P
Molecule details ›
Chain: P
Length: 100 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-100; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
precursor form of the Hepatitis Delta virus ribozyme Chain: R
Molecule details ›
Chain: R
Length: 76 nucleotides
Theoretical weight: 24.47 KDa
Source organism: Hepatitis delta virus
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R32
Unit cell:
a: 108.566Å b: 108.566Å c: 190.319Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.26 0.25 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli