› 1sm2

X-ray diffraction
2.3Å resolution

Crystal structure of the phosphorylated Interleukin-2 tyrosine kinase catalytic domain

Released:
DOI: 10.2210/pdb1sm2/pdb
PDB entry 1sm2 coloured by chain, front view.
PDB entry 1sm2 coloured by chain, side view.
PDB entry 1sm2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of interleukin-2 tyrosine kinase and their implications for the design of selective inhibitors.
Brown K, Long JM, Vial SC, Dedi N, Dunster NJ, Renwick SB, Tanner AJ, Frantz JD, Fleming MA, Cheetham GM
J Biol Chem 279 18727-32 (2004)
PMID: 14766749

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ITK/TSK Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08881 (Residues: 357-620; Coverage: 43%)
Gene names: EMT, ITK, LYK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 2 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: C2
Unit cell:
a: 125.1Å b: 74.4Å c: 78.9Å
α: 90° β: 93.9° γ: 90°
R-values:
R R work R free
0.246 0.214 0.292
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Tec family kinases in T lymphocyte development and function.
Berg et al. (2005)
9 more

8 mentions without citation

Structures of human Bruton's tyrosine kinase in active and inactive conformations suggest a mechanism of activation for TEC family kinases.
Marcotte et al. (2010)
7 more