› 1sra

X-ray diffraction
2Å resolution

STRUCTURE OF A NOVEL EXTRACELLULAR CA2+-BINDING MODULE IN BM-40(SLASH)SPARC(SLASH)OSTEONECTIN

Released:
DOI: 10.2210/pdb1sra/pdb
PDB entry 1sra coloured by chain, front view.
PDB entry 1sra coloured by chain, side view.
PDB entry 1sra coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a novel extracellular Ca(2+)-binding module in BM-40.
Hohenester E, Maurer P, Hohenadl C, Timpl R, Jansonius JN, Engel J
Nat Struct Biol 3 67-73 (1996)
PMID: 8548457

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPARC Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 17.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P09486 (Residues: 153-303; Coverage: 53%)
Gene names: ON, SPARC
Sequence domains: Secreted protein acidic and rich in cysteine Ca binding region
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 54.7Å b: 55.2Å c: 75.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.249
Expression system: Homo sapiens

Quick links

Citations

22 review citations

Macromolecular organization of basement membranes.
Timpl R. (1996)
21 more

16 mentions without citation

Improved prediction of critical residues for protein function based on network and phylogenetic analyses.
Thibert et al. (2005)
15 more