› 1sy6

X-ray diffraction
2.1Å resolution

Crystal Structure of CD3gammaepsilon Heterodimer in Complex with OKT3 Fab Fragment

Released:
DOI: 10.2210/pdb1sy6/pdb
PDB entry 1sy6 coloured by chain, front view.
PDB entry 1sy6 coloured by chain, side view.
PDB entry 1sy6 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3.
Kjer-Nielsen L, Dunstone MA, Kostenko L, Ely LK, Beddoe T, Mifsud NA, Purcell AW, Brooks AG, McCluskey J, Rossjohn J
Proc Natl Acad Sci U S A 101 7675-80 (2004)
PMID: 15136729

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-209330 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
OKT3 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.84 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: Q6PJA7 (Residues: 20-128, 129-242; Coverage: 48%)
Gene name: Igh
Sequence domains:
Structure domains: Immunoglobulins
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09693 (Residues: 23-103; Coverage: 51%)
  • Canonical: P07766 (Residues: 21-118; Coverage: 52%)
Gene names: CD3E, CD3G, T3E, T3G
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 67.702Å b: 55.77Å c: 96.05Å
α: 90° β: 100.85° γ: 90°
R-values:
R R work R free
0.211 0.211 0.255
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

24 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
23 more

32 mentions without citation

Structural biology of the T-cell receptor: insights into receptor assembly, ligand recognition, and initiation of signaling.
Wucherpfennig et al. (2010)
31 more