› 1t08

X-ray diffraction
2.1Å resolution

Crystal structure of beta-catenin/ICAT helical domain/unphosphorylated APC R3

Released:
DOI: 10.2210/pdb1t08/pdb
PDB entry 1t08 coloured by chain, front view.
PDB entry 1t08 coloured by chain, side view.
PDB entry 1t08 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of phosphorylation-dependent binding of APC to beta-catenin and its role in beta-catenin degradation.
Ha NC, Tonozuka T, Stamos JL, Choi HJ, Weis WI
Mol Cell 15 511-21 (2004)
PMID: 15327768

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150341 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Catenin beta-1 Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 56.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35222 (Residues: 146-664; Coverage: 67%)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Beta-catenin-interacting protein 1 Chain: B
Molecule details ›
Chain: B
Length: 46 amino acids
Theoretical weight: 5.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NSA3 (Residues: 8-53; Coverage: 57%)
Gene names: CTNNBIP1, ICAT
Sequence domains: Beta-catenin-interacting protein ICAT
Structure domains: Beta-catenin-interacting ICAT
Adenomatous polyposis coli protein Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25054 (Residues: 1484-1498; Coverage: 1%)
Gene names: APC, DP2.5
Sequence domains: APC repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, null
Spacegroup: P21212
Unit cell:
a: 96.472Å b: 96.712Å c: 86.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.204 0.241
Expression system: Escherichia coli BL21

Quick links

Citations

59 review citations

Wnt/beta-catenin signaling: components, mechanisms, and diseases.
MacDonald et al. (2009)
58 more

4 mentions without citation

In silico structure-based approaches to discover protein-protein interaction-targeting drugs.
Shin et al. (2017)
3 more