› 1t8u

X-ray diffraction
1.95Å resolution

Crystal Structure of human 3-O-Sulfotransferase-3 with bound PAP and tetrasaccharide substrate

Released:
DOI: 10.2210/pdb1t8u/pdb
PDB entry 1t8u coloured by chain, front view.
PDB entry 1t8u coloured by chain, side view.
PDB entry 1t8u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the sulfotransferase (3-o-sulfotransferase isoform 3) involved in the biosynthesis of an entry receptor for herpes simplex virus 1.
Moon AF, Edavettal SC, Krahn JM, Munoz EM, Negishi M, Linhardt RJ, Liu J, Pedersen LC
J Biol Chem 279 45185-93 (2004)
PMID: 15304505

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-195337 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heparan sulfate glucosamine 3-O-sulfotransferase 3A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 31.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y663 (Residues: 139-406; Coverage: 66%)
Gene names: 3OST3A1, HS3ST3A, HS3ST3A1, UNQ2551/PRO6180
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

Carbohydrate polymer : NEW Components: SGN, IDS, UAP
Carbohydrate polymer
3 bound ligands:
Ligand SO4 1 x SO4
Ligand NA 2 x NA
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2221
Unit cell:
a: 80.806Å b: 154.525Å c: 91.931Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.222
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Glycomics approach to structure-function relationships of glycosaminoglycans.
Sasisekharan et al. (2006)
14 more

14 mentions without citation

Heparan sulfate 3-O-sulfation: a rare modification in search of a function.
Thacker et al. (2014)
13 more