› 1t91

X-ray diffraction
1.9Å resolution

crystal structure of human small GTPase Rab7(GTP)

Released:
DOI: 10.2210/pdb1t91/pdb
PDB entry 1t91 coloured by chain, front view.
PDB entry 1t91 coloured by chain, side view.
PDB entry 1t91 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for recruitment of RILP by small GTPase Rab7.
Wu M, Wang T, Loh E, Hong W, Song H
EMBO J 24 1491-501 (2005)
PMID: 15933719

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rab-7a Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 23.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51149 (Residues: 1-207; Coverage: 100%)
Gene names: RAB7, RAB7A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand GTP 4 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P1
Unit cell:
a: 56.695Å b: 56.777Å c: 74.51Å
α: 71.51° β: 71.72° γ: 77.75°
R-values:
R R work R free
0.252 0.243 0.247
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The cytoplasmic dynein transport machinery and its many cargoes.
Reck-Peterson et al. (2018)
12 more

22 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
21 more