› 1tag

X-ray diffraction
1.8Å resolution

STRUCTURAL DETERMINANTS FOR ACTIVATION OF THE ALPHA-SUBUNIT OF A HETEROTRIMERIC G PROTEIN

Released:
DOI: 10.2210/pdb1tag/pdb
PDB entry 1tag coloured by chain, front view.
PDB entry 1tag coloured by chain, side view.
PDB entry 1tag coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein.
Lambright DG, Noel JP, Hamm HE, Sigler PB
Nature 369 621-8 (1994)
PMID: 8208289

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 27-350; Coverage: 93%)
Gene name: GNAT1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 71.9Å b: 87.8Å c: 143.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.211
Expression system: Not provided

Quick links

Citations

79 review citations

Heterotrimeric G proteins: organizers of transmembrane signals.
Neer EJ. (1995)
78 more

33 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
32 more