› 1tat

X-ray diffraction
3Å resolution

CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS

Released:
DOI: 10.2210/pdb1tat/pdb
PDB entry 1tat coloured by chain, front view.
PDB entry 1tat coloured by chain, side view.
PDB entry 1tat coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Crystalline mitochondrial aspartate aminotransferase exists in only two conformations.
Hohenester E, Jansonius JN
J Mol Biol 236 963-8 (1994)
PMID: 8120903

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 44.99 KDa
Source organism: Gallus gallus
Expression system: unidentified
UniProt:
  • Canonical: P00508 (Residues: 23-423; Coverage: 95%)
Gene name: GOT2
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 69.5Å b: 89.6Å c: 144.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 not available not available
Expression system: unidentified

Quick links

Citations

2 review citations

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han et al. (2010)
1 more

7 mentions without citation

Cysteine S-conjugate β-lyases: important roles in the metabolism of naturally occurring sulfur and selenium-containing compounds, xenobiotics and anticancer agents.
Cooper et al. (2011)
6 more