› 1tjt

X-ray diffraction
2.19Å resolution

X-ray structure of the human alpha-actinin isoform 3 at 2.2A resolution

Released:
DOI: 10.2210/pdb1tjt/pdb
PDB entry 1tjt coloured by chain, front view.
PDB entry 1tjt coloured by chain, side view.
PDB entry 1tjt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the actin binding domain from alpha-actinin in its closed conformation: structural insight into phospholipid regulation of alpha-actinin.
Franzot G, Sjöblom B, Gautel M, Djinović Carugo K
J Mol Biol 348 151-65 (2005)
PMID: 15808860

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin-3 Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08043 (Residues: 26-273; Coverage: 28%)
Gene name: ACTN3
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P21
Unit cell:
a: 46.31Å b: 38.54Å c: 65.48Å
α: 90° β: 99.72° γ: 90°
R-values:
R R work R free
0.227 0.227 0.273
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Filamins: promiscuous organizers of the cytoskeleton.
Popowicz et al. (2006)
13 more

8 mentions without citation

Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating.
Engler et al. (2008)
7 more