› 1tup

X-ray diffraction
2.2Å resolution

TUMOR SUPPRESSOR P53 COMPLEXED WITH DNA

Released:
DOI: 10.2210/pdb1tup/pdb
PDB entry 1tup coloured by chain, front view.
PDB entry 1tup coloured by chain, side view.
PDB entry 1tup coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a p53 tumor suppressor-DNA complex: understanding tumorigenic mutations.
Cho Y, Gorina S, Jeffrey PD, Pavletich NP
Science 265 346-55 (1994)
PMID: 8023157

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114659 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 219 amino acids
Theoretical weight: 24.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-312; Coverage: 56%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
DNA (5'-D(*TP*TP*TP*CP*CP*TP*AP*GP*AP*CP*TP*TP*GP*CP*CP*CP*A P*AP*TP*TP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*TP*AP*AP*TP*TP*GP*GP*GP*CP*AP*AP*GP*TP*CP*TP*A P*GP*GP*AP*A)-3') Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 117.5Å b: 67.9Å c: 108.8Å
α: 90° β: 105.5° γ: 90°
R-values:
R R work R free
0.202 0.202 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

313 review citations

p53, the cellular gatekeeper for growth and division.
Levine AJ. (1997)
312 more

69 mentions without citation

Why are there hotspot mutations in the TP53 gene in human cancers?
Baugh et al. (2018)
68 more