› 1u0f

X-ray diffraction
1.6Å resolution

Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate

Released:
DOI: 10.2210/pdb1u0f/pdb
PDB entry 1u0f coloured by chain, front view.
PDB entry 1u0f coloured by chain, side view.
PDB entry 1u0f coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening.
Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C
J Mol Biol 342 847-60 (2004)
PMID: 15342241

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 564 amino acids
Theoretical weight: 63.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06745 (Residues: 1-558; Coverage: 100%)
Gene names: Gpi, Gpi1
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand G6P 1 x G6P
Ligand G6Q 1 x G6Q
Ligand SO4 8 x SO4
Ligand GOL 12 x GOL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 69.4Å b: 116.8Å c: 73.9Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.18 0.178 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Anticancer agents that counteract tumor glycolysis.
Granchi et al. (2012)
24 other articles

6 mentions without citation

A novel N-terminal domain may dictate the glucose response of Mondo proteins.
McFerrin et al. (2012)
5 more