› 1u0g

X-ray diffraction
1.7Å resolution

Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate

Released:
DOI: 10.2210/pdb1u0g/pdb
PDB entry 1u0g coloured by chain, front view.
PDB entry 1u0g coloured by chain, side view.
PDB entry 1u0g coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening.
Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C
J Mol Biol 342 847-60 (2004)
PMID: 15342241

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 564 amino acids
Theoretical weight: 63.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06745 (Residues: 1-558; Coverage: 100%)
Gene names: Gpi, Gpi1
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 7 x SO4
Ligand E4P 2 x E4P
Ligand BME 5 x BME
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 68.8Å b: 115.6Å c: 73.2Å
α: 90° β: 100.2° γ: 90°
R-values:
R R work R free
0.192 0.19 0.215
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Anticancer agents that counteract tumor glycolysis.
Granchi et al. (2012)
25 other articles

1 mention without citation

Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv.
Anand et al. (2010)