› 1u0n

X-ray diffraction
2.95Å resolution

The ternary von Willebrand Factor A1-glycoprotein Ibalpha-botrocetin complex

Released:
DOI: 10.2210/pdb1u0n/pdb
PDB entry 1u0n coloured by chain, front view.
PDB entry 1u0n coloured by chain, side view.
PDB entry 1u0n coloured by chain, top view.
Source organisms:
Primary publication:
The snake venom protein botrocetin acts as a biological brace to promote dysfunctional platelet aggregation.
Fukuda K, Doggett T, Laurenzi IJ, Liddington RC, Diacovo TG
Nat Struct Mol Biol 12 152-9 (2005)
PMID: 15665869

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137565 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
von Willebrand factor Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04275 (Residues: 1261-1468; Coverage: 8%)
Gene names: F8VWF, VWF
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Snaclec botrocetin subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 15.23 KDa
Source organism: Bothrops jararaca
UniProt:
  • Canonical: P22029 (Residues: 1-133; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Snaclec botrocetin subunit beta Chain: C
Molecule details ›
Chain: C
Length: 125 amino acids
Theoretical weight: 15.05 KDa
Source organism: Bothrops jararaca
UniProt:
  • Canonical: P22030 (Residues: 1-125; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Glycocalicin Chain: D
Molecule details ›
Chain: D
Length: 265 amino acids
Theoretical weight: 29.49 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07359 (Residues: 17-281; Coverage: 42%)
Gene name: GP1BA
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 108.315Å b: 108.315Å c: 221.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.276
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

Quick links

Citations

9 review citations

Haemostatically active proteins in snake venoms.
Sajevic et al. (2011)
8 more

16 mentions without citation

Animal toxins - Nature's evolutionary-refined toolkit for basic research and drug discovery.
Herzig et al. (2020)
15 more