› 1u1p

X-ray diffraction
1.9Å resolution

Crystal Structure of UP1 Complexed With d(TTAGGGTTA 2PR GG); A Human Telomeric Repeat Containing 2-aminopurine

Released:
DOI: 10.2210/pdb1u1p/pdb
PDB entry 1u1p coloured by chain, front view.
PDB entry 1u1p coloured by chain, side view.
PDB entry 1u1p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human UP1 as a model for understanding purine recognition in the family of proteins containing the RNA recognition motif (RRM).
Myers JC, Shamoo Y
J Mol Biol 342 743-56 (2004)
PMID: 15342234

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114674 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09651 (Residues: 1-196; Coverage: 53%)
Gene names: HNRNPA1, HNRPA1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-D(*T*TP*AP*GP*GP*GP*TP*TP*AP*(2PR)P*GP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.76 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 2PR 1 x 2PR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 51.701Å b: 51.701Å c: 171.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Sequence-specific binding of single-stranded RNA: is there a code for recognition?
Auweter et al. (2006)
3 more

5 mentions without citation

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
4 more