› 1u3v

X-ray diffraction
1.65Å resolution

Crystal Structure of Human Alcohol Dehydrogenase Beta-1-Beta-1 Isoform Complexed with N-Heptylformamide Determined to 1.65 Angstrom Resolution

Released:
DOI: 10.2210/pdb1u3v/pdb
PDB entry 1u3v coloured by chain, front view.
PDB entry 1u3v coloured by chain, side view.
PDB entry 1u3v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of three class I human alcohol dehydrogenases complexed with isoenzyme specific formamide inhibitors.
Gibbons BJ, Hurley TD
Biochemistry 43 12555-62 (2004)
PMID: 15449945

Function and Biology Details

Reaction catalysed: 
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
All-trans-retinol dehydrogenase [NAD(+)] ADH1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00325 (Residues: 2-375; Coverage: 100%)
Gene names: ADH1B, ADH2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand ZN 4 x ZN
Ligand HPL 2 x HPL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P1
Unit cell:
a: 44.32Å b: 52.83Å c: 90.35Å
α: 79.58° β: 89.43° γ: 68.6°
R-values:
R R work R free
0.165 0.164 0.185
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Conformational changes and catalysis by alcohol dehydrogenase.
Plapp BV. (2010)
1 more

4 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
3 more