› 1u3w

X-ray diffraction
1.45Å resolution

Crystal Structure of Human Alcohol Dehydrogenase Gamma-2-Gamma-2 Isoform Complexed with N-1-Methylheptylformamide Determined to 1.45 Angstrom Resolution

Released:
DOI: 10.2210/pdb1u3w/pdb
PDB entry 1u3w coloured by chain, front view.
PDB entry 1u3w coloured by chain, side view.
PDB entry 1u3w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of three class I human alcohol dehydrogenases complexed with isoenzyme specific formamide inhibitors.
Gibbons BJ, Hurley TD
Biochemistry 43 12555-62 (2004)
PMID: 15449945

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase 1C Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00326 (Residues: 2-375; Coverage: 100%)
Gene names: ADH1C, ADH3
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand FXY 2 x FXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 55.361Å b: 67.125Å c: 92.725Å
α: 90° β: 103.75° γ: 90°
R-values:
R R work R free
0.185 0.184 0.211
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
2 more

7 mentions without citation

Atomic-resolution structures of horse liver alcohol dehydrogenase with NAD(+) and fluoroalcohols define strained Michaelis complexes.
Plapp et al. (2012)
6 more