› 1u6g

X-ray diffraction
3.1Å resolution

Crystal Structure of The Cand1-Cul1-Roc1 Complex

Released:
DOI: 10.2210/pdb1u6g/pdb
PDB entry 1u6g coloured by chain, front view.
PDB entry 1u6g coloured by chain, side view.
PDB entry 1u6g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Cand1-Cul1-Roc1 complex reveals regulatory mechanisms for the assembly of the multisubunit cullin-dependent ubiquitin ligases.
Goldenberg SJ, Cascio TC, Shumway SD, Garbutt KC, Liu J, Xiong Y, Zheng N
Cell 119 517-28 (2004)
PMID: 15537541

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158747 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cullin-1 Chain: A
Molecule details ›
Chain: A
Length: 776 amino acids
Theoretical weight: 89.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13616 (Residues: 1-776; Coverage: 100%)
Gene name: CUL1
Sequence domains:
Structure domains:
E3 ubiquitin-protein ligase RBX1 Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62877 (Residues: 1-108; Coverage: 100%)
Gene names: RBX1, RNF75, ROC1
Sequence domains: RING-H2 zinc finger domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Cullin-associated NEDD8-dissociated protein 1 Chain: C
Molecule details ›
Chain: C
Length: 1230 amino acids
Theoretical weight: 136.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86VP6 (Residues: 1-1230; Coverage: 100%)
Gene names: CAND1, KIAA0829, TIP120, TIP120A
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 108.466Å b: 151.327Å c: 215.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.243 0.317
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

50 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
49 more

31 mentions without citation

Anticancer drug discovery by targeting cullin neddylation.
Yu et al. (2020)
30 more