› 1u7t

X-ray diffraction
2Å resolution

Function and Biology Details

Reactions catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH
Testosterone + NAD(+) = androstenedione + NADH
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-CoA dehydrogenase type-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 261 amino acids
Theoretical weight: 27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99714 (Residues: 1-261; Coverage: 100%)
Gene names: ERAB, HADH2, HSD17B10, MRPP2, SCHAD, SDR5C1, XH98G2
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand TDT 3 x TDT

Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 122Å b: 80.8Å c: 110Å
α: 90° β: 105.6° γ: 90°
R-values:
R R work R free
0.215 0.215 0.263
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The chemistry of Alzheimer's disease.
Rauk A. (2009)
12 more

12 mentions without citation

Virtual screening applications in short-chain dehydrogenase/reductase research.
Beck et al. (2017)
11 more