› 1u81

Solution NMR

Delta-17 Human ADP Ribosylation Factor 1 Complexed with GDP

Released:
DOI: 10.2210/pdb1u81/pdb
PDB entry 1u81 coloured by chain, ensemble of 11 models, front view.
PDB entry 1u81 coloured by chain, ensemble of 11 models, side view.
PDB entry 1u81 coloured by chain, ensemble of 11 models, top view.
Source organism: Homo sapiens
Primary publication:
Conformational changes in human Arf1 on nucleotide exchange and deletion of membrane-binding elements.
Seidel RD, Amor JC, Kahn RA, Prestegard JH
J. Biol. Chem. 279 48307-18 (2004)
PMID: 15308674

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 1 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84077 (Residues: 18-181; Coverage: 91%)
Gene name: ARF1
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Cartesian Min (No RDC), Torsion Angle Min (Ramp RDC), Cartesian Min (Ramp RDC), Cartesian Min (Ro RDC), Torsion Angle Min (RDC)
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Viral membrane scission.
Rossman et al. (2013)
11 other articles

5 mentions without citation

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Cui et al. (2016)
4 more